Abstract

DO.12.10

Alkylphosphocholines as possible Inhibitors of posterior capsule opacification after cataract surgery

Liegl R., Kernt M., Kollias A., Kampik A., Priglinger S., Eibl K. H.
Augenklinik der LMU München, Campus Innenstadt, München

Objective: Posterior capsule opacification (PCO) is known as the major complication of otherwise uneventful cataract surgery within the first 5 postoperative years. PCO incidence varies with the type of intraocular lens (IOL) implanted between 2 % for hydrophobic acrylates/silicone IOLs and up to 100 % for accommodative and multifocal IOLs. In addition, Nd:YAG-Capsulotomy induces a decrease in accommodative capacity of these IOLs. Therefore, the possibility of PCO prevention by a single-dose pharmacologic agent administered intraoperatively has long been sought. The aim if this in vitro study was to investigate the effect of Alkylphosphocholines (APCs) on human lens epithelial cell proliferation.
Methods: The immortalized human lens epithelial cell line HLE-B3 was incubated for 24 hrs with an APC in different concentrations in the presence of DMEM / 20 % FCS under standard cell culture conditions. To assess toxic effects, the Live/Dead Assay was performed. The cells were then incubated at the maximal slope of the growth curve, after 72 hrs, with APCs and the cellular proliferation was measured with the MTT Test 24 hrs later. The IC₅₀ concentration was determined.
Results: APCs can effectively inhibit human lens epithelial cell proliferation in a dose-dependent manner. The IC₅₀ concentration is 200 mikroM ±SD in the presence of 20 % FCS. The Live/Dead Assay did not show any significant difference compared to controls in the concentration interval tested.
Conclusions: APCs are effective inhibitors of human lens epithelial cell proliferation in vitro in non-toxic concentrations. They have the potential for a topic application as a single-dose agent. This might represent a novel option for pharmacologic PCO prevention. However, further studies are needed before a clinical application can be performed.

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